|
KMID : 0545119980080060625
|
|
Journal of Microbiology and Biotechnology
1998 Volume.8 No. 6 p.625 ~ p.630
|
|
|
Construction of a Secretory Expression Vector Porducing an α-Amylase of Yeast, Schwanniomyces occidentalis in Saccharomyces
|
|
Shin, Dong Jun
Park, Jong Chun/Lee, Hwang hee/Chun, Soon Bai/Bai, Suk
|
|
|
Abstract
|
|
|
|
Using a modified yeast secretory expression vector, α-amylase of Schwanniomyces occidentalis was produced from Saccharomyces cerevisiae. The expression vector contains the α-amylase gene (AMY) harboring its own promoter without the regulatory region and the adenine base at the -3 position from the ATG start codon, its own signal sequence, CYC1 transcription terminator, and SV40 enhancer. The expressed α-amylase activity from cells carrying the plasmid was approximately 26 times higher than that from the cells harboring an unmodified plasmid. When Saccharomyces diastaticus was transformed with this modified vector, a 2.5 times higher level of amylolytic activity than that from Sch. occidentalis was observed.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|